2D40 Research Cluster Shared Equipment list

Biorad Bioplex 200

The Biorad Bioplex 200 Multiplex Immunoassay System utilizes Luminex xMAP technology to enable the simultaneous detection and quantification of multiple analytes in small volumes. Multiplex immunoassays use Luminex magnetic beads for the quantification biologically relevant targets. Users are responsible for their own assay kits and reagents.

This instrument is located in 2D40.

Please contact Heather Neufeld at heather.neufeld@usask.ca for training.

The booking calendar for this item can be found here.

Sorvall RC-6 Centrifuge

The Ovsenek Lab's Sorval RC-6 High Speed Refrigerated Centrifuge has the following fixed angle rotors:

F-20/MICRO       (32 X 1.5 mL microtubes; Maximum Speed 20,000 rpm; Maximum RCF 51,427)

SS-34                (8 X 50 mL tubes; Maximum Speed 20,000 rpm - Maximum RCF 47,808)

SLA-1500           (6 X 250 mL capped bottles; Maximum Speed 14,500 rpm - Maximum RCF 31,916)

This instrument is located in 2D40.3.

Please contact Heather Neufeld at heather.neufeld@usask.ca for training and booking of this instrument.

The booking calendar for this item can be found here.

CPX-130 Ultrasonic Processor

The Desai Lab's ultrasonic disruptor has programmable timer and pulse control. The unit's 3mm titanium probe can process volumes between 250 uL and 10 mL.

This instrument is located in 2D40.3.8.

Please contact Heather Neufeld at heather.neufeld@usask.ca for training to book this instrument.

The booking calendar for this item can be found here.

Tissue Homogenizer

The lab has a number of manually controlled larger wand homogenizers (1 cm and greater in diameter). These units are suitable for larger tissue samples, and can process samples in volumes of >2 mL.

The instruments are located in 2D40.3.8.

BMG NOVOstar Microplate Fluorometer

The Gordon Lab Lab's NOVOstar microplate fluorometer can perform plate-to-plate transfers with three reagent stations. Incubation, shaking, user-defined kinetic sampling rates, and bottom reading can be performed. Both absorbance- and fluorescence-based applications can be carried out using the provided wavelength filters.

This instrument is located in 2D40.

Please contact John Gordon or Heather Neufeld at john.gordon@usask.ca or heather.neufeld@usask.ca for filter information and training on this instrument.

There is currently no booking calendar for this instrument - please contact us to confirm availability.

VWR 1585 Orbital Shaking Incubator

The Liu Lab's incubating shaker has racks for 15 mL, 50 mL tubes and smaller (<1 L) culture flasks. It is kept at 37 decrees Celsius with a maximum speed of 250 RPM.

This instrument is located in 2D40.3.8.

Please contact Lixin Liu and Heather Neufeld at lixin.liu@usask.ca or heather.neufeld@usask.ca for training and permission to use this instrument.