BSL2+ Tissue Culture facility
BSL2+ Tissue Culture facility for specialized research needs.
Contact linda.chelico@usask.ca
Equipment List
Beckman Coulter Avanti JXN-26 High Speed Centrifuge
High Speed Centrifuge
Contact linda.chelico@usask.ca
Biorad CFX96 Real-Time PCR Detection System
Biorad Real-Time PCR Detection System (C1000 Thermocycler)
Contact joyce.wilson@usask.ca
Biorad Gene Pulser Xcell Electroporation System
Cell electroporation System
Contact Juveriya Khan in the Wilson Lab jqk913@mail.usask.ca
Biorad ZOE Fluorescent Cell Imager
Fluorescent Cell Imaging System
Contact Juveriya Khan in the Wilson Lab jqk913@mail.usask.ca
Eppendorf™ Mastercycler™ pro PCR System
Thermocycler
Contact Juveriya Khan in the Wilson Lab jqk913@mail.usask.ca
Fluorometer
Fluorometer for fluorescence deopolarization, total fluorescence or FRET. Cuvette format.
Contact linda.chelico@usask.ca
GloMax® 20/20 Luminometer
Ultrasensitive Single Tube Luminometer
Contact Juveriya Khan in the Wilson Lab jqk913@mail.usask.ca
Biorad Chemidoc System
Biorad Chemidoc System
For training, contact Adi Manek at adi.manek@usask.ca
ELISpot Reader
Zeiss LSM700 Confocal Microscope
- ***NEW - recently relocated in the 6th floor Health Sciences Research Cluster - Room B632. Still use this booking calendar until one is set up on the 6th floor Health Sciences Research Cluster website
- View Booking Calendar here
- User fees apply, $30/hr
- immunofluorescence microscopy focused on one 'layer' of the cell
- can build 3-D images by taking multiple Z-stacks
- coimmunofluorescence and colocalization using software analysis
- fixed or live cells imaging
- video imaging capability to capture imaging over time
- multiple fluores can be used in the same sample and the same time
- GFP. RFP, CFP. YFP, DAPI, brightfield
- Alexa dyes, Cy dyes
- spectral unmixing capabilities, even for dyes with overlapping spectra
- FRET, FRAP experiments
- significant training required
Purchased with funds from CFI, the College of Medicine, University of Saskatachewan and the Saskatchewan Cancer Agency
For training, contact Stephanie at stephanie.kendall@usask.ca