Want to learn a new technique or take advantage of equipment with training provided?
Simply contact the expert user identified in this table and arrange a time to receive this training.
Database last updated: November, 2019.
| Technique | Procedure | Quantitative | Equipment | Primary Contact | Lab | ||
| Apoptosis | Annexin V | Yes | Beckman CytoFlex Flow Cytometer | Mark Boyd | Lab Manager | mark.boyd@usask.ca | |
| Bacterial Protein Expression | Cloning, Mutagenesis and Protein Extraction/Purification | No |
Thermo MaxQ Shaking Incubator, Pharmacia Biotech Ultraspec 3000 Spectrophotometer, Centrifuge (Beckman J2-21M, J2-MI) |
Paul Mellor | Anderson | paul.mellor@usask.ca | |
| Cell-based Assays | Adhesion | Yes | Paul Mellor | Anderson | paul.mellor@usask.ca | ||
| Chemotaxis/Migration | Yes | Paul Mellor | Anderson | paul.mellor@usask.ca | |||
| Foci Formation | Yes | Paul Mellor | Anderson | paul.mellor@usask.ca | |||
| Hypoxia | Yes | Hypoxic Chamber | Paul Mellor | Anderson | paul.mellor@usask.ca | ||
| In-cell Western | Yes | LICOR Odyssey Infrared Scanner | Paul Mellor | Anderson | paul.mellor@usask.ca | ||
| Invasion/Wound Healing | Yes | Paul Mellor | Anderson | paul.mellor@usask.ca | |||
| MTT Assay | Yes | Molecular Devices Spectra Max M5 Plate Reader | Paul Mellor | Anderson | paul.mellor@usask.ca | ||
| Non-adherant Growth (soft agar assay) | Yes | Paul Mellor | Anderson | paul.mellor@usask.ca | |||
| Cell cycle assay | Cell cycle assay, fluorescence-based | Yes | Beckman CytoFlex Flow Cytometer | Mark Boyd | Lab Manager | mark.boyd@usask.ca | |
| Confocal Microscopy | Fixed-cell Imaging, Live Cell Imaging, Z-stacks | No | Zeiss LSM 700 Confocal Microscope | Mark Boyd | Lab Manager | mark.boyd@usask.ca | |
| Co-localization | Yes | Zeiss LSM 700 Confocal Microscope | Mark Boyd | Lab Manager | mark.boyd@usask.ca | ||
| DNA Quantification | Flow Cytometry (intact cells) | Yes | Beckman CytoFlex Flow Cytometer | Mark Boyd | Lab Manager | mark.boyd@usask.ca | |
| NanoDrop (extracted DNA) | Yes | Nanodrop 2000C | Paul Mellor | Anderson | paul.mellor@usask.ca | ||
| DNA Sequencing | PCR Product Template | No | Applied Biosystems 2720 or Veriti Thermocycler | Paul Mellor | Anderson | paul.mellor@usask.ca | |
| Plasmid Template | No | Paul Mellor | Anderson | paul.mellor@usask.ca | |||
| Methylation Sequencing (bisulfite treatment) | No | Applied Biosystems 2720 or Veriti Thermocycler | Stephanie Kendall | Anderson | scj503@mail.usask.ca | ||
| Drying | Speed Vacuum | No | Labconco Centrivap Speed Vacuum | Mark Boyd | Lab Manager | mark.boyd@usask.ca | |
| Enzyme Assays | kinetic assays | Yes | GloMax® 96-well microplate luminometer w/ dual injectors (Promega) | Mark Boyd | Lab Manager | mark.boyd@usask.ca | |
| dual gene reporter assays | Yes | GloMax® 96-well microplate luminometer w/ dual injectors (Promega) | Mark Boyd | Lab Manager | mark.boyd@usask.ca | ||
| luciferase assays | Yes | GloMax® 96-well microplate luminometer w/ dual injectors (Promega) | Mark Boyd | Lab Manager | mark.boyd@usask.ca | ||
| Cell viability/ATP assays | Yes | GloMax® 96-well microplate luminometer w/ dual injectors (Promega) | Mark Boyd | Lab Manager | mark.boyd@usask.ca | ||
| Intracellular Ca+2 assays | Yes | GloMax® 96-well microplate luminometer w/ dual injectors (Promega) | Mark Boyd | Lab Manager | mark.boyd@usask.ca | ||
| Immunoassays | Yes | GloMax® 96-well microplate luminometer w/ dual injectors (Promega) | Mark Boyd | Lab Manager | mark.boyd@usask.ca | ||
| Flow cytometry | Immunodetection of up to 10 colors | Yes | CytoFLEX V2-B5-R3 Flow Cytometer (10 Detectors, 3 Lasers) | Mark Boyd | Lab Manager | mark.boyd@usask.ca | |
| Fluorescence | Fluorescent Gel Imaging (protein and DNA) | Yes | BioRad ChemiDoc MP Imaging System | Mark Boyd | Lab Manager | mark.boyd@usask.ca | |
| Fluorescent Western Blot Imaging | Yes | BioRad ChemiDoc MP Imaging System | Mark Boyd | Lab Manager | mark.boyd@usask.ca | ||
| Yes | Varioskan Microplate Reader | Tanya Freywald | Freywald (4D10) | tanya.freywald@usask.ca | |||
| Gene Expression Analysis | Microarray analysis | Yes | Frederick Vizeacoumar | Vizeacoumar | frederick.vizeacoumar@usask.ca | ||
| Gene Knockdown | shRNA libraries, individual genes or pooled | shRNAs, each gene in human genome, 3-5 shRNA/gene; & pooled; lentiviral | Frederick Vizeacoumar | Vizeacoumar | frederick.vizeacoumar@usask.ca | ||
| High-throughput imaging | Fluorescence imaging in 96-well format, 384-well format | No | ImageXpress Micro XLS Widefield and Ultra Confocal High-Content Microscopes | Frederick Vizeacoumar | Vizeacoumar | frederick.vizeacoumar@usask.ca | |
| Fluorescence imaging 96-well, 384-well format | Yes | CellInsight CX7 | Paul Mellor | Anderson | paul.mellor@usask.ca | ||
| Immunohisto Chemistry | Slide Preparation, Probing | No | Stephanie Kendall | Anderson | scj503@mail.usask.ca | ||
| Imaging | Semi | EVOS5000 | Stephanie Kendall | Anderson |
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| Microarray | deconvolution of synthetic lethal screen data | Yes | Frederick Vizeacoumar | Vizeacoumar | frederick.vizeacoumar@usask.ca | ||
| PCR | Primer Design, PCR Optimization (including using gradient system) | No | Primer3 Software, Applied Biosystems 2720 Thermocycler, Veriti gradient thermocycler | Paul Mellor | Anderson | paul.mellor@usask.ca | |
| Agarose Gel Elecrophoresis and Gel Imaging | No | Gel Beds, VWR Accu Power 300 Power Supply; BioRad Gel Doc, Quanti One software | Stephanie Kendall | Anderson | scj503@mail.usask.ca | ||
| PCR, Digital Droplet | Copy Number Variation, Mutation Detection, Quantitation | Yes | BioRad QX100 Droplet Generator and Droplet Reader (Lab 2D10) | Brooke Thompson | Kirychuk (2D10) | bnt576@mail.usask.ca | |
| qPCR, Real-time | RNA Extraction, Reverse Transcription, Real-time PCR and Data Analysis | Yes | Applied Biosystems 2720 or Veriti Thermocycler, then Applied Biosystems StepOne Plus instrument & software | Stephanie Kendall | Anderson | scj503@mail.usask.ca | |
| Protein Quantificaiton | Lowry (lysate | Yes | Pharmacia Biotech Ultraspec 3100 Pro or Spectrophotometer, Molecular Devices Spectra Max M5 Plate Reader | Stephanie Kendall | Anderson | scj503@mail.usask.ca | |
| ELISA | Yes | Variskan Lux Microplate Reader | Mark Boyd | Lab Manager | mark.boyd@usask.ca | ||
| Flow Cytometry (intact cells) | Yes | CytoFLEX V2-B5-R3 Flow Cytometer (10 Detectors, 3 Lasers) | Mark Boyd | Lab Manager | mark.boyd@usask.ca | ||
| Radio Isotope Assays | ATPase Assay | Yes | Beckman LS 6000IC Scintillation Counter | Hao Ding | Wu | dih078@mail.usask.ca | |
| EMSA | Yes | Beckman LS 6000IC Scintillation Counter | Hao Ding | Wu | dih078@mail.usask.ca | ||
| Helicase Assay | Yes | Beckman LS 6000IC Scintillation Counter | Manhong Guo | Wu | mag919@mail.usask.ca | ||
| GAP Assay | Semi | Deborah Anderson | Anderson | deborah.anderson@saskcancer.ca | |||
| Kinase Assay | Semi | Deborah Anderson | Anderson | deborah.anderson@saskcancer.ca | |||
| Detection | Yes | Typhoon Phosphorimager | Wu Lab | ||||
| Surface Wipe Test | Yes | Beckman LS 6000IC Scintillation Counter, in room B128 | Mark Boyd | Lab Manager | mark.boyd@usask.ca | ||
| Reporter Assays | Luminometer | Yes | GloMax® 96-well microplate luminometer w/ dual injectors (Promega) | Mark Boyd | Lab Manager | mark.boyd@usask.ca | |
| Western Blot | Protein Extraction (whole cell lysate) | >No |
|
Anderson | scj503@mail.usask.ca | ||
| SDS-PAGE | No | BioRad gel tanks, power packs | Dielle Whitecross | Anderson | ded442@mail.usask.ca | ||
| Transfer (wet) | No | BioRad Mini Protean II | Lukong lab | Lukong | |||
| Transfer (semi-dry) | No | Fisher Semi-Dry Blotting Unit FB-SDB-2020 | Deborah Anderson | Anderson | deborah.anderson@saskcancer.ca | ||
| Transfer (semi-dry), antibody probing and detection (LICOR-Infrared) | No | BioRad Trans-Blot Turbo Transfer System, LICOR Odyssey | Stephanie Kendall | Anderson | scj503@mail.usask.ca | ||
| Detection (chemiluminescence) | No | BioRad ChemiDoc MP Imaging System | Lukong Lab | Lukong | |||