Shared Equipment

To arrange access to the Cancer Cluster instruments please contact:

Mark Boyd Lab Manager of 4th floor, and 6th Floor  Office Room 4B30 Phone: (306) 370-1296 E-mail: mark.boyd@usask.ca

See Expert User Database for contact information for an expert user that can provide the necessary training needed to use the equipment for the particular application needed.

If the data obtained using the Cancer Cluster instrumentation are used for publication, please include the statement below in the acknowledgements section:

"Research described in this paper was performed in part at the Cancer Research Cluster, which is supported by the Saskatchewan Cancer Agency and the College of Medicine, University of Saskatchewan."

Zeiss LSM700 system

• ***NEW - recently relocated in the 6th floor Health Sciences Research Cluster - Room B632. Still use this booking calendar until one is set up on the 6th floor Health Sciences Research Cluster website
• View Booking Calendar here
• User fees apply, $30/hr
• immunofluorescence microscopy focused on one 'layer' of the cell
• can build 3-D images by taking multiple Z-stacks
• coimmunofluorescence and colocalization using software analysis
• fixed or live cells imaging
• video imaging capability to capture imaging over time
• multiple fluores can be used in the same sample and the same time
• GFP. RFP, CFP. YFP, DAPI, brightfield
• Alexa dyes, Cy dyes
• spectral unmixing capabilities, even for dyes with overlapping spectra
• FRET, FRAP experiments
• significant training required

Purchased with funds from CFI, the College of Medicine, University of Saskatachewan and the Saskatchewan Cancer Agency.

Beckman CytoFLEX Flow Cytometer

• View Booking Calendar here
• User fees apply, $50/hr
• 3 lasers; 488 nm, 638 nm, 405 nm
• capable of detecting 13 colors at once
• 96-plates or single samples
• Quantification of cell surface markers or intracellular proteins
• Requires antibodies to target protein
• Cell cycle analysis
• Apoptosis assay

• More info here: https://www.beckman.com/flow-cytometry/instruments/cytoflex

Purchased and maintained with funds from the SCA 

EVOS 5000 and Celleste Analysis Software workstation

• View Booking Calendar here
• User-defined fluorescence, transmitted light, and color applications
• Autofocus, Z-stack capability, time-lapse imaging, and single-click multi-channel capture
• On-board software for acquisition, analysis, and automated cell counting
• Light cubes: DAPI, GFP, TX RED
• Objectives: 4X, 10X, 20X, 40X, 60X
• Wide variety of sample holders for slides and various sizes of plates/dishes

Celleste Image Analysis software available to use on-site at computer workstation beside EVOS5000.

Purchased and maintained with funds from the SCA 

Olympus IX51

• immunofluorescence microscopy
• green, red, blue, brightfield
• e.g. GFP, RFP, DAPI, light images
• photography capability
• permission of owner Dr. Lukong required

BioSperix - ProOx Model 110

• senses and displaces oxygen on an ongoing basis
• requires user supplied nitrogen tank
• oxygen gas sensor can be set to maintain desired oxygen level
• fits inside existing CO2 incubator

Purchased with funds from the SCA 

ChemiDoc MP Imaging System - 17001402

• View Booking Calendar here
• multiplex fluorescent western blotting
• chemiluminescence detection
• general gel documentation applications and stain-free technology
• fluorescent reporters (RGB, far red, and near infrared) including StarBright™ Fluorophores
• detect up to three proteins simultaneously
• features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images
• stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins
• three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
• White Sample Tray, for gels stained with Coomassie Blue, copper, silver, or zinc stains

• Trans-UV, 302 nm 
  Epi-white
  Trans-white (White Sample Tray required)
  Trans-blue, 450-490 nm 
  Epi-blue, 460-490 nm excitation
  Epi-green, 520-545 nm excitation
  Epi-red, 625-650 nm excitation
  Epi-far red, 650-675 nm excitation; so suitable for IR-680 dyes (like LICOR)
  Epi-near IR, 755-777 nm excitation; so suitable for IR-800 dyes (like LICOR)

Note: an identical BioRad Chemidoc system is also available to use and is located in the Common Equipment Core Facility in B128, HSc here.

Purchased with funds from the SCA 

The IVIS^® Spectrum in vivo imaging system combines 2D optical and 3D optical tomography in one platform. The system uses leading optical technology for preclinical imaging research and development ideal for non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.

Features and Benefits

• High-sensitivity in vivo imaging of fluorescence and bioluminescence
• High throughput (5 mice) with 23 cm field of view
• High resolution (to 20 microns) with 3.9 cm field of view
• Twenty eight high efficiency filters spanning 430 – 850 nm
• Supports spectral unmixing applications
• Ideal for distinguishing multiple bioluminescent and fluorescent reporters
• Optical switch in the fluorescence illumination path allows reflection-mode or transmission-mode illumination
• 3D diffuse tomographic reconstruction for both fluorescence and bioluminescence

• Booking calendar coming soon
• Live cell imaging e.g., proliferation, or cytotoxicity assays
• Leave your cells undisturbed in the incubator during image acquisition
• Multiple imaging modes – HD phase plus red and green fluorescence
• 4x, 10x and 20x objectives
• Accommodates up to six microplates at a time
• Users can schedule experiments at different image acquisition frequencies and magnifications in parallel
• Remote, networked access with unlimited, free licenses
• Incucyte compatible vessels can be found here
• Incucyte handbook can be found here

Purchased with funds from the Cancer Foundation of Saskatchewan. Maintained with funds from the SCA.

• View Booking Calendar here
• quantitative Western/Immunoblots for protein detection
• much less expensive than chemiluminescence (no film, no chemi solutions)
• uses infrared-linked secondary antibodies (very economical)
• directly scans blots to provide accurate quantification over several orders of magnitude
• more sensitive detection than film (from chemi)
• digital image of same quality as film (from chemi)
• two channels to detect two species of antibodies at the same time (e.g. mouse and rabbit)
• in-cell Western to carry out higher throughput analyses in 96-well format for validated antibodies that only recognize one protein in cells

Note: an similar LICOR Odyssey scanner system is also available to use and is located in the Common Equipment Core Facility in B128, HSc here.

Purchased and maintained with funds from the SCA 

GloMax(R) 96 Microplate Luminometer w/ Dual Injectors from Promega

• View Booking Calendar here
• chemiluminescent and bioluminescent applications
• highly sensitive and quantitative; nine logs of dynamic range
• capable of performing both flash and glow-type luminescent assays
• injector volume range from 25 - 250 ul in 1-ul increments
• software wizards assist with protocol development
• Suitable for: dual reporter assays, steady-Glo® Luciferase Assays, Flash Glow Luciferase Assays, Cell Viability/ATP Assays, Kinetic Assays, intracellular Ca+2 Assays, Immunoassays
• Spectral Range: 350-650 nm
• Detection Limit: 3 x 10-21 moles of luciferase
• Requires the use of 96-well microplates
• Data exports into Excel spreadsheet

Purchased with funds from the SCA 

Varioskan Lux Multimode Microplate Reader

• View Booking Calendar here
• Absorbance (UV-Vis, including pathlength correction)
• Fluorescence Intensity (including FRET)
• Luminescence (direct and filtered, including BRET)
• AlphaScreen / AlphaLISA

• The instrument selects the measurement wavelength using filters or monochromators, depending on which is optimal for each measurement technology.

  • Monochromators in absorbance and fluorescence intensity
  • Filters in AlphaScreen and time-resolved fluorescence
  • Luminescence without wavelength selection (filters can be used if required)

• Absorbance wavelength range: 200 - 1000 nm
• Light Source: Xenon Flash Lamp
• Fluorescence excitation wavelength range: 200 - 1000 nm
• Fluorescence emission wavelength range: 270 - 840 nm
• Luminescence wavelength range: 360 - 670 nm
• Measurment modes: End-point, kinetic, spectra, multipoint and kinetic spectra

Purchased and maintained with funds from the SCA 

Nanodrop 2000C

• View Booking Calendar here
• determination of protein or nucleic acid concentrations
• uses very small sample sizes

Purchased with funds from the SCA 

The Next-Generation Sequencing Facility (NGSF) is a high-throughput genomics facility located at the University of Saskatchewan. It is supported by the Saskatchewan Cancer Agency and University of Saskatchewan, College of Medicine. The primary goal of the NGSF is to further basic and clinical research by providing access to high-throughput sequencing technologies and expertise.

In addition to library preparation and sequencing services, data analysis and bioinformatics services are also available, either together or each independently.

Visit the main NGSF page for more information, including a list of shared equipment (Qubit, Tapestation, qPCR, iSeq, NextSeq and more). Contact us to discuss your project objectives and obtain a service quote.

• NGSF
• ngsf@usask.ca

• gene knockdown (shRNA) and gene knockout (CRISPR) technology
• high-content screening and imaging capabilities
• User fees apply
• ImageXpress Micro XLS Widefield High Content Screening System
• ImageXpress Ultra Confocal High-Content Analysis System
• Capable of fluorescence imaging of 96-well plates
• Pooled shRNA library, lentiviral delivery
• Arrayed shRNA library, lentiviral delivery
• CRISPR-Cas9 gene editing tehcnology
• More information can be found here.

Typhoon FLA 7000, from GE Healthcare

• View Booking Calendar here
• Typhoon FLA7000 Scanner Main Bi-Alkali PMT Laser
  LD 635 Laser LD 650 Laser LD 473 Laser SHG 532 Y520 filter (FLA7k) O580 filter (FLA7k) R670
  filter (FLA7k) IP filter (FLA7k) Fluor stage (FLA7k) Membrane weight (FLA7k) Phosphor stage
  (FLA7k) Fluorescent plate Capture software
• digital detection and quantification of 32P and 35S-based radioactive assays on gels and/or TLC plates; requires high resolution phosphor screens to capture information. Some shared and available for use from Deborah Anderson (deborah.anderson@saskcancer.ca)
• Fluorescent gel imaging of DNA and Protein
• Fluorescent Western blot imaging

Purchased with funds from the SCA 

Applied Biosystems StepOne Plus

• View Booking Calendar here
• quantitative mRNA expression level measurements
• Users must purchase the appropriate reagents that are compatible with this machine, specifically these exact 96-well plates and seals:

Note: an identical Applied Biosystems StepOne Plus system is also available to use and is located in the Common Equipment Core Facility in B128, HSc here.

Purchased with funds from the College of Medicine, University of Saskatchewan     

• to dry samples under vacuum in 1.5 mL Eppendorf sized tubes

Optima L-100 XP Ultracentrifuge

• very high speed centrifugation, under vacuum

Purchased and maintained with funds from the SCA 

RTCA DP Model from ACEA Biosciences

• Live cell real time analyses with multiple measurements over hours and days
• Equipment resides in CO2 incubator
• Cell proliferation measurements
• Cell cytotoxicity measurements (e.g. IC50 determinations)
• Cell migration/invasion measurements
• Morphology change measurements
• specialized 16 well plates with electrodes, that collects data for each of 3 plates independently
• different users can use one of the 3 cradles with independent data collection parameters
• each of the 3 cradles holding the plates must be reserved/booked ahead of time since expts run over multiple days
• measures cell impedance with no dyes or additives needed
• cells can be recovered and analyzed further (e.g. immunoblot or immunofluorescence)

Purchased with funds from the SCA