Access to Equipment

To arrange access to the Cancer Cluster instruments please contact:

Mark Boyd
Lab Manager of 4th floor, and 6th Floor 
Office Room 4B30
Phone: (306) 370-1296
E-mail: mark.boyd@usask.ca

See Expert User Database for contact information for an expert user that can provide the necessary training needed to use the equipment for the particular application needed.

If the data obtained using the Cancer Cluster instrumentation are used for publication, please include the statement below in the acknowledgements section:


"Research described in this paper was performed in part at the Cancer Research Cluster, which is supported by the Saskatchewan Cancer Agency and the College of Medicine, University of Saskatchewan."

Confocal Microscope

Zeiss LSM700 system

  • View Booking Calendar here
  • User fees apply, $30/hr
  • immunofluorescence microscopy focused on one 'layer' of the cell
  • can build 3-D images by taking multiple Z-stacks
  • coimmunofluorescence and colocalization using software analysis
  • fixed or live cells imaging
  • video imaging capability to capture imaging over time
  • multiple fluores can be used in the same sample and the same time
  • GFP. RFP, CFP. YFP, DAPI, brightfield
  • Alexa dyes, Cy dyes
  • spectral unmixing capabilities, even for dyes with overlapping spectra
  • FRET, FRAP experiments
  • significant training required

Purchased with funds from CFI, the College of Medicine, University of Saskatachewan and the Saskatchewan Cancer Agency. Maintained with funds from the SCA.

InnovationCoMSCA

Flow Cytometer

Beckman CytoFLEX Flow Cytometer

  • View Booking Calendar here
  • User fees apply, $50/hr
  • 3 lasers; 488 nm, 638 nm, 405 nm
  • capable of detecting 10 colors at once
  • 96-plates or single samples
  • Quantification of cell surface markers or intracellular proteins
  • Requires antibodies to target protein
  • Cell cycle analysis
  • Apoptosis assay
  • CytoFLEX V2-B5-R3 Flow Cytometer (10 Detectors, 3 Lasers)
  • More info here: https://www.beckman.com/flow-cytometry/instruments/cytoflex

Purchased and maintained with funds from the SCA SCA logo

Fluorescent Microscope System (EVOS 5000)

EVOS 5000 and Celleste Analysis Software workstation

  • View Booking Calendar here
  • User-defined fluorescence, transmitted light, and color applications
  • Autofocus, Z-stack capability, time-lapse imaging, and single-click multi-channel capture
  • On-board software for acquisition, analysis, and automated cell counting
  • Light cubes: DAPI, GFP, TX RED
  • Objectives: 4X, 10X, 40X, 60X, 100X
  • Wide variety of sample holders for slides and various sizes of plates/dishes

Celleste Image Analysis software available to use on-site at computer workstation beside EVOS5000.

Purchased and maintained with funds from the SCA SCA logo

Fluorescence Microscope System (Olympus IX51)

Olympus IX51

  • immunofluorescence microscopy
  • green, red, blue, brightfield
  • e.g. GFP, RFP, DAPI, light images
  • photography capability
  • permission of owner Dr. Lukong required

Hypoxia Chamber

BioSperix - ProOx Model 110

  • senses and displaces oxygen on an ongoing basis
  • requires user supplied nitrogen tank
  • oxygen gas sensor can be set to maintain desired oxygen level
  • fits inside existing CO2 incubator

Purchased with funds from the SCA SCA logo

Imaging System - BioRad

ChemiDoc MP Imaging System - 17001402

  • View Booking Calendar here
  • multiplex fluorescent western blotting
  • chemiluminescence detection
  • general gel documentation applications and stain-free technology
  • fluorescent reporters (RGB, far red, and near infrared) including StarBright™ Fluorophores
  • detect up to three proteins simultaneously
  • features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images
  • stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins
  • three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
  • White Sample Tray, for gels stained with Coomassie Blue, copper, silver, or zinc stains
  • Trans-UV, 302 nm 
    Epi-white
    Trans-white (White Sample Tray required)
    Trans-blue, 450-490 nm 
    Epi-blue, 460-490 nm excitation
    Epi-green, 520-545 nm excitation
    Epi-red, 625-650 nm excitation
    Epi-far red, 650-675 nm excitation; so suitable for IR-680 dyes (like LICOR)
    Epi-near IR, 755-777 nm excitation; so suitable for IR-800 dyes (like LICOR)

Note: an identical BioRad Chemidoc system is also available to use and is located in the Common Equipment Core Facility in B128, HSc here.

Purchased with funds from the SCA SCA logo

LICOR CLx Odyssey Scanner

  • View Booking Calendar here
  • quantitative Western/Immunoblots for protein detection
  • much less expensive than chemiluminescence (no film, no chemi solutions)
  • uses infrared-linked secondary antibodies (very economical)
  • directly scans blots to provide accurate quantification over several orders of magnitude
  • more sensitive detection than film (from chemi)
  • digital image of same quality as film (from chemi)
  • two channels to detect two species of antibodies at the same time (e.g. mouse and rabbit)
  • in-cell Western to carry out higher throughput analyses in 96-well format for validated antibodies that only recognize one protein in cells

Note: an similar LICOR Odyssey scanner system is also available to use and is located in the Common Equipment Core Facility in B128, HSc here.

Purchased and maintained with funds from the SCA SCA logo

Luminometer

GloMax(R) 96 Microplate Luminometer w/ Dual Injectors from Promega

  • View Booking Calendar here
  • chemiluminescent and bioluminescent applications
  • highly sensitive and quantitative; nine logs of dynamic range
  • capable of performing both flash and glow-type luminescent assays
  • injector volume range from 25 - 250 ul in 1-ul increments
  • software wizards assist with protocol development
  • Suitable for: dual reporter assays, steady-Glo® Luciferase Assays, Flash Glow Luciferase Assays, Cell Viability/ATP Assays, Kinetic Assays, intracellular Ca+2 Assays, Immunoassays
  • Spectral Range: 350-650 nm
  • Detection Limit: 3 x 10-21 moles of luciferase
  • Requires the use of 96-well microplates
  • Data exports into Excel spreadsheet

Purchased with funds from the SCA SCA logo

Microplate Reader

Varioskan Lux Multimode Microplate Reader

  • View Booking Calendar here
  • Absorbance (UV-Vis, including pathlength correction)
  • Fluorescence Intensity (including FRET)
  • Luminescence (direct and filtered, including BRET)
  • AlphaScreen / AlphaLISA
  • The instrument selects the measurement wavelength using filters or monochromators, depending on which is optimal for each measurement technology.

    • Monochromators in absorbance and fluorescence intensity
    • Filters in AlphaScreen and time-resolved fluorescence
    • Luminescence without wavelength selection (filters can be used if required)
  • Absorbance wavelength range: 200 - 1000 nm
  • Light Source: Xenon Flash Lamp
  • Fluorescence excitation wavelength range: 200 - 1000 nm
  • Fluorescence emission wavelength range: 270 - 840 nm
  • Luminescence wavelength range: 360 - 670 nm
  • Measurment modes: End-point, kinetic, spectra, multipoint and kinetic spectra

Purchased and maintained with funds from the SCA SCA logo

Nanodrop

Nanodrop 2000C

Purchased with funds from the SCA SCA logo

Next-Generation Sequencing Facility

The Next-Generation Sequencing Facility (NGSF) is a high-throughput genomics facility located at the University of Saskatchewan. It is supported by the Saskatchewan Cancer Agency and University of Saskatchewan, College of Medicine. The primary goal of the NGSF is to further basic and clinical research by providing access to high-throughput sequencing technologies and expertise.

In addition to library preparation and sequencing services, data analysis and bioinformatics services are also available, either together or each independently.

Visit the main NGSF page for more information, including a list of shared equipment (Qubit, Tapestation, qPCR, iSeq, NextSeq and more). Contact us to discuss your project objectives and obtain a service quote.

CoM      SCA logo

Phenogenomic Imaging Centre of Saskatchewan

  • gene knockdown (shRNA) and gene knockout (CRISPR) technology
  • high-content screening and imaging capabilities
  • User fees apply
  • ImageXpress Micro XLS Widefield High Content Screening System
  • ImageXpress Ultra Confocal High-Content Analysis System
  • Capable of fluorescence imaging of 96-well plates
  • Pooled shRNA library, lentiviral delivery
  • Arrayed shRNA library, lentiviral delivery
  • CRISPR-Cas9 gene editing tehcnology
  • More information can be found here.

Innovation  UofS

Phosphorimager

Typhoon FLA 7000, from GE Healthcare

  • View Booking Calendar here
  • Typhoon FLA7000 Scanner Main Bi-Alkali PMT Laser
    LD 635 Laser LD 650 Laser LD 473 Laser SHG 532 Y520 filter (FLA7k) O580 filter (FLA7k) R670
    filter (FLA7k) IP filter (FLA7k) Fluor stage (FLA7k) Membrane weight (FLA7k) Phosphor stage
    (FLA7k) Fluorescent plate Capture software
  • digital detection and quantification of 32P and 35S-based radioactive assays on gels and/or TLC plates; requires high resolution phosphor screens to capture information. Some shared and available for use from Deborah Anderson (deborah.anderson@saskcancer.ca)
  • Fluorescent gel imaging of DNA and Protein
  • Fluorescent Western blot imaging

Purchased with funds from the SCA SCA logo

Real-Time PCR

Applied Biosystems StepOne Plus

  • View Booking Calendar here
  • quantitative mRNA expression level measurements
  • Users must purchase the appropriate reagents that are compatible with this machine, specifically these exact 96-well plates and seals:

qPCR platesqPCR seals

Note: an identical Applied Biosystems StepOne Plus system is also available to use and is located in the Common Equipment Core Facility in B128, HSc here.

Purchased with funds from the College of Medicine, University of Saskatchewan     

CoM

Speed Vac

  • to dry samples under vacuum in 1.5 mL Eppendorf sized tubes

Ultracentrifuge

Optima L-100 XP Ultracentrifuge

  • very high speed centrifugation, under vacuum

Purchased and maintained with funds from the SCA SCA logo

xCelligence

RTCA DP Model from ACEA Biosciences

  • Live cell real time analyses with multiple measurements over hours and days
  • Equipment resides in CO2 incubator
  • Cell proliferation measurements
  • Cell cytotoxicity measurements (e.g. IC50 determinations)
  • Cell migration/invasion measurements
  • Morphology change measurements
  • specialized 16 well plates with electrodes, that collects data for each of 3 plates independently
  • different users can use one of the 3 cradles with independent data collection parameters
  • each of the 3 cradles holding the plates must be reserved/booked ahead of time since expts run over multiple days
  • measures cell impedance with no dyes or additives needed
  • cells can be recovered and analyzed further (e.g. immunoblot or immunofluorescence)

Purchased with funds from the SCA SCA logo